When preparing your campaign, the first step is to select the audience. By clicking on the 'Advanced' button, you can choose the included audience and the excluded audience:

Without the need to create a specific segment beforehand, in this example we show how to select customers who have made at least one purchase, excluding recent contacts who have made a purchase.

Note: This functionality is only available in the Premium plan. If you don't have this plan, it will be necessary to create the desired segment beforehand by applying the corresponding filters.